In recent years, a lot of development has been observed in this field. A variety of such biosensors are being used for various purposes. e.g. there are amperometric biosensors for the determination of glycolic acid in real samples, few of them are based on the immobilization of enzymes in polymer films electrogenerated from a series of amphiphilic pyrrole derivatives, for apparent direct electron transfer between electrodes and immobilized peroxidases, selective ones for glucose byÂ in situÂ electropolymerization, screen-printed amperometric biosensors for the rapid measurement of L- and D-amino acids and flow injection analysis of L-lactate in milk and yoghurt by on-line micro dialysis and amperometric detection at a disposable biosensor etc.2
The significance of amperometric biosensors which determine glycolic acid in real samples lies in the fact that they are the first enzyme based biosensors which have a lot of potential. They have a power to find out the occurrence of glycolic acid in complex matrix samples such as instant coffee, cosmetics, and urine. It made the work of analysts a lot easier as two methods for this were devised and they were according to the current technology of commercial analyzers.
Both the designs had three-membrane configuration consisting of an inner and an outer membrane3. The inner membrane is cellulose acetate membrane (CA) and the outer membrane is polycarbonate membrane (PC). Both of them comprise of a membrane that contains biomolecule(s).
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