When a spleen tissue section is stained using Congo red, amyloid appears to be orange red when observed through normal light. However, when viewed under light that is polarized, it imparts an apple green birefringence to the material (Jones, Hunt and King, 1997, p. 50). However, the nuclei appear blue. This is a property that all Î² â€“ pleated proteins tend to share and it seems to be particular to that given conformation (Bancroft and Gomble, 2008, p. 273).
â€œAmyloid is the deposition within tissues of a group of pathological proteins which form characteristic Î² pleated sheetsâ€ (McCarthy, Hacking and Al Mufti, 2004, p. 155). It comprises of a fibrillar ultrastructure and serum protein A and P are the proteins that constitute it. It is also comprised of immunoglobulin light chains and peptide hormones. It may either be local or systemic and it does interfere with the tissue or organ that has been affected, normally through the accumulation of the basement membrane.
Histologic staining using Congo red is what characterizes all forms of amyloid; hence this remains a requirement for diagnosing the disease (Gertz and Greipp, 2004, p. 158).
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