Glycolysis is a process that results in the fission of a six-carbon sugar into a three-carbon acid, pyruvate ion. The experiment was designed to investigate the impact of temperature on the rate of Glycolysis. The substrate was prepared from 775 grams ofÂ Saccharomyces cerevisaeÂ that was mixed with 50 grams of glucose, a hexose sugar. Four test tubes were labeled depending on the temperature of the corresponding water bath namely control, cold, warm and hot. The rate of Glycolysis was measured as a function of carbon dioxide gas that was evolved within a thirty-minute time frame. The average carbon dioxide evolved was determined as well as t-test values. The experiment results demonstrated that the rate of Glycolysis increases as temperature increases which resulted in the acceptance of hypothesis 1 and rejection of hypothesis 2.
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