This experiment tested a number of aspects that arise from DNA extraction. It touches on plant cell DNA extraction, animal cell DNA extraction, sequence used in DNA extraction and composition of the sample. This leads us to the hypothesis of the experiment which states that â€œDNA yield is grossly affected by the sequence of extraction, DNA source components and tissue source.â€
DNA isolation involves extraction of DNA from various sources (Albert et al 35). Little literature elaborates the differences between DNA extractions from different sources (Simon, Reece and Dickey 11). While researchers have suggested that plant cells produce more DNA than animal cells, others argue that DNA production differs with individual sources and not as plant or animal category (Albert et al 35). Additionally, it is important to note that research has indicated that DNA is only present in living organisms and can therefore not be found in non-living organism (Albert et al 35). Despite the fact that there exist various methods for extraction of DNA, ultimately they all bear similarity and perform similar steps (Keroin 20). Both aim at separation of DNA from other cellular components. Often presence of other substances like proteins, lipids, polysaccharides and other compounds are likely to interfere with the analysis methods used and are likely to interfere with DNA yield and purity. Likewise difference in composition of plant and animal cells is believed to have increased likelihood of affecting DNA yield and purity upon extraction (Lerner and Brenda 1).
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