The first procedure was to launch the software and lower pedestal with a soft wipe on the computer. The objective of this was to enable proper reporting. A blank sample was pipetted into the lower pedestal while the sampling arm was open. The sample chamber was closed by lowering the swing arm. On the screen, a click on the blank page launched analysis of the sample (Slater 2009). To reduce the probability of unreliable data, the process was repeated two times. On the cycle, a sample containing the extracted DNA was placed on the lower pedestal that was 1ul and the chamber closed.
After the sample was measured and the data printed, a procedure was carried out to determine the concentration. It was analysed through the use of a spectrophotometer to gauge its absorbance at 260nm (A260). In addition, A280 and A230 were measured. Ratios of A260 to A230 and those of A260 to A280 were used to measure the quality of DNA. The quality of this genetic material was measured by adjusting the A260 by absorbance at 320nm. The product of this was multiplied by the dilution factor. It was done through the use of A260 of 1.0=50ug/ml pure dsDNA.
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