This experiment was conducted in the histopathology laboratory to illustrate routine staining by use of special stains. The special stains used were the Massonâ€™s trichrome and Congo red. The Massonâ€™s trichrome is commonly used to reveal the architecture of tissues and in the preparation of frozen sections. This is carried out systematically using the necessary specimen that entails mainly paraffin sections. Congo red detects amyloid in tissues of patients with amyloidosis. Massonâ€™s trichrome is for carrying out experiments that can help in the determination of collagen in tissues such as skin and heart for analysis and likely diagnosis.
Itâ€™s also used to draw a distinction between smooth muscle and collagen in tumors. The Massonâ€™s trichrome stain was used in the staining of normal uterus and the uterus fibroid. The former turned pinkish red while the latter turned red-blue. On the other hand, Congo red stained a section of the spleen tissue where its components turned colorless and light pink. Methods of carrying frozen sections were illustrated. This was followed by staining of the slide and examination using the microscope. There was the fine adjustment of the microscope to help give a clear image from which the specimenâ€™s diagram was observed.
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