To the PBS were added protein inhibitors. Thereafter 200 ml pellet was resuspended in 1oml PBS with 100 Î¼l lysozyme enzyme. The contents were then incubated at room temperature for 15 minutes on the rotary suspension mixer. Thereafter, the contents were freeze-thawed 7 times, first using liquid nitrogen, then in a water bath, at 37Â oC. Then, 100 Î¼l DNase enzymes were added, and the contents incubated on the rotary suspension mixer for 15 minutes. Spinning then followed at 12,000rpm for 30 min, at a speed of 4oC. The supernatant and the pellet were then placed in separate tubes and the sample loaded on SDS page.
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